In Vitro Fertilized Embryos do not Secrete Detectable HLA-G on Day Two

in vitro fertilized embryos do not secrete detectable hla-g on day two

background: pregnancy is a successful transplantation. the factors evading rejection of the fetus are poorly understood. recently an interest has grown, in hla-g molecules as one of these factors. since these antigens are mainly expressed on the surface of cytotro-phoblasts that are in direct contact with maternal tissues, it has been suggested that these molecules may have a role in induction ...

P-203: Investigating Association of HLA-G Gene Polymorphisms and Failed Implantation in Human Embryos

Background: HLA-G is a non-classical human leukocyte antigen expressed primarily in fetal tissues at the maternal-fetal interface. The unique structure of HLA-G molecule permits a restricted peptide presentation and allows the modulation of the immune system. There is now general agreement that HLA-G is an important immunotolerant molecule with the capability of inhibiting immune cell functions...

Sex-related differences in the developmental rate of in-vitro matured/in-vitro fertilized ovine embryos.

Sex determination of in-vitro matured/in-vitro fertilized ovine embryos cultured in synthetic oviduct fluid (SOF) medium was performed by the polymerase chain reaction amplification of specific Y DNA sequences so as to test the influence of sex on developmental growth during the preimplantation period. At 144 h post-insemination, embryos with a blastocoel were classified as the fast-developing ...

Stimulation of development of in vitro-matured and in vitro-fertilized bovine embryos by platelets.

In vitro-fertilized bovine embryos were incubated in Menezo's B2 medium (MB2) supplemented with 2 mg/mL of BSA. In Exp. 1, eight-cell stage embryos were allotted to one of the following groups: control medium (MB2), MB2 with 20 ng/mL of platelet-activating factor (PAF), 1 x 10(7) bovine blood platelets (Platelets), oviductal cells (BOEC), BOEC and 20 ng/mL of PAF (BOEC+PAF), or BOEC and 1 x 10(...

Effects of Antioxidants on Development of In Vitro Fertilized Bovine Embryos

The soluble pool of HLA-G produced by human trophoblasts does not include detectable levels of the intron 4-containing HLA-G5 and HLA-G6 isoforms.

In the context of implantation and pregnancy, several immunomodulating functions have been attributed to the different HLA-G isoforms. Increasing attention is now being addressed to the actively secreted soluble forms, because they might have a systemic function or could be useful as diagnostic tools. However, the cellular source of secretion, even during pregnancy, where HLA-G expression level...